MMS19 Polyklonaler Antikörper

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• KD/KO Validated

MMS19 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 16015-1-AP

Synonyme

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

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Galerie der Validierungsdaten

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  WB analysis using 16015-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16015-1-AP (MMS19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16015-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16015-1-AP (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16015-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16015-1-AP (MMS19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• CoIP: See 1 publications below

Produktinformation

16015-1-AP bindet in WB, IF, CoIP, ELISA MMS19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMS19 fusion protein Ag8792
• Vollständiger Name: MMS19 nucleotide excision repair homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 1030 aa, 113 kDa
• Beobachtetes Molekulargewicht: 103-113 kDa
• GenBank-Zugangsnummer: BC006575
• Gene symbol: MMS19
• Gene ID (NCBI): 64210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMS19 plays a critical role in the biogenesis of multiple Fe-S proteins, which functions in genomic stability, RNA polymerase II function, and telomere length regulation. MMS19 has a alanine- and leucine-rich N terminus. It possible has a role in nucleotide excision repair(NER) and RNA polymerase II(POL II) transcription by interacting with ERCC2/XPB helicases, both subunits of NER-transcription factor TFIIH. Also MMS19 involves in the regulation of ER. It can function in chromosome segregation by acting as a part of the mitotic spindle-associated MMXD complex.

Publikationen

Species	Application	Title
human	WB	Cell; Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.; Authors - Tslil Ast
human	WB	Mol Cell; Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.; Authors - Jenny L Weon ; KD Validated
human	WB	Cell Metab; Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.; Authors - Stehling Oliver O ; KD Validated
mouse	WB	Nat Commun; Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases.; Authors - Seung Wook Yang
human	WB	Cell Rep; The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.; Authors - Diana C Odermatt ; KD Validated
mouse	WB	Mol Metab; Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue.; Authors - Colin J Palmer