MMP7 Monoklonaler Antikörper

MMP7 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E6D6

Kat-Nr. : 67990-1-Ig

Synonyme

Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 67990-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67990-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67990-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 67990-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 67990-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 67990-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human pancreas cancer using 67990-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human pancreas cancer using 67990-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human pancreas cancer using 67990-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, A549-Zellen, COLO 320-Zellen, HT-29-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Pankreaskarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67990-1-Ig bindet in WB, IHC, IF, ELISA MMP7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MMP7 fusion protein Ag0550
• Vollständiger Name: matrix metallopeptidase 7 (matrilysin, uterine)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC003635
• Gene symbol: MMP7
• Gene ID (NCBI): 4316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.