MMP21 Polyklonaler Antikörper

MMP21 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 55289-1-AP

Synonyme

matrix metallopeptidase 21, Matrix metalloproteinase 21, MMP 21, MMP21

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 55289-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55289-1-AP (MMP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55289-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55289-1-AP (MMP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 55289-1-AP

  Immunofluorescent analysis of L02 cells using 55289-1-AP (MMP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, L02-Zellen
• Erfolgreiche Detektion in IF: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

55289-1-AP bindet in WB, IHC, IF, ELISA MMP21 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: matrix metallopeptidase 21
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: NM_147191
• Gene symbol: MMP21
• Gene ID (NCBI): 118856
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP21 belongs to the peptidase M10A family. It may have an important and specific function in tumor progression and embryogenesis. It cleaves alpha-1-antitrypsin. The proenzyme without the signal peptide has a calculated molecular mass of 62 kD; following activation by furin, the processed enzyme has a calculated molecular mass of 49 kD(PMID:12617721),and MMP21 protein was detected in fetal neuroectoderm, in placenta, and in leukocytes(OMIM).This antibody is specific to MMP21.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Wnt activation disturbs cell competition and causes diffuse invasion of transformed cells through NF-κB-MMP21 pathway; Authors - Kazuki Nakai
human	WB	Hum Genomics; De novo disruptive heterozygous MMP21 variants are potential predisposing genetic risk factors in Chinese Han heterotaxy children; Authors - Xi-Ji Qin
human,mouse	WB,IHC	Cancer Sci; Matrix metalloproteinase-21 promotes metastasis via increasing the recruitment and M2 polarization of macrophages in HCC; Authors - Jiangfan Zhou ; KD Validated