MMP19 Polyklonaler Antikörper

MMP19 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14244-1-AP

Synonyme

matrix metallopeptidase 19, Matrix metalloproteinase 18, Matrix metalloproteinase 19, Matrix metalloproteinase RASI, MMP 18, MMP 19, MMP18, MMP19, RASI, RASI 1

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 14244-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14244-1-AP (MMP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14244-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14244-1-AP (MMP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14244-1-AP

  IP Result of anti-MMP19 (IP:14244-1-AP, 4ug; Detection:14244-1-AP 1:500) with human placenta tissue lysate 1520ug.

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  IHC staining of human liver cancer using 14244-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14244-1-AP (MMP19 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 14244-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14244-1-AP (MMP19 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of SH-SY5Y using 14244-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using MMP19 antibody (14244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

14244-1-AP bindet in WB, IP, IHC, IF, ELISA MMP19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP19 fusion protein Ag5501
• Vollständiger Name: matrix metallopeptidase 19
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-60 kDa
• GenBank-Zugangsnummer: BC050368
• Gene symbol: MMP19
• Gene ID (NCBI): 4327
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP19(matrix metalloproteinase-19) has the capacity to degrade several basement membrane proteins such as type IV collagen, laminin, tenascin C, and nidogen. It is expressed by microvascular endothelial cells in vitro as well as by capillaries in the inflamed synovial membrane, whereas its expression is absent in quiescent macrovascular endothelial cells of large arteries and veins . And it is also detected in capillaries of normal mammary tissue, benign mammary carcinomas and in skin capillaries(PMID:15241558).

Publikationen

Species	Application	Title
mouse	WB	Cancer Gene Ther; A critical ETV4/Twist1/Vimentin axis in Ha-RAS-induced aggressive breast cancer.; Authors - Wuling Liu
human	WB,IHC	J Cell Biochem; MicroRNA-193b-3p Regulates Matrix Metalloproteinase 19 Expression in Interleukin-1β-Induced Human Chondrocytes.; Authors - Zong-Kun Chang
human	WB	Front Pharmacol; METTL3/m6A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma; Authors - Fangfang Ge
human	WB	J Biochem Mol Toxicol; Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling; Authors - Wei Xu
rat	WB	J Immunol Res; Dissecting the Molecular Mechanism of Wang-Bi Capsule in the Treatment of Experimental Rheumatoid Arthritis Based on Synovial Tissue Proteomic Analysis.; Authors - Haiyang Shu