MMP12 Polyklonaler Antikörper

MMP12 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Rezensionen (3)

Kat-Nr. : 22989-1-AP

Synonyme

HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12

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Galerie der Validierungsdaten

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  WB analysis using 22989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22989-1-AP (MMP12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22989-1-AP (MMP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22989-1-AP (MMP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Lungengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 11 publications below
• IF: See 8 publications below

Produktinformation

22989-1-AP bindet in WB, IHC, IF, ELISA MMP12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Kaninchen, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP12 fusion protein Ag19068
• Vollständiger Name: matrix metallopeptidase 12 (macrophage elastase)
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa, 45 kDa
• GenBank-Zugangsnummer: BC112301
• Gene symbol: MMP12
• Gene ID (NCBI): 4321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).

Publikationen

Species	Application	Title
mouse	IF	Nat Cell Biol; Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence; Authors - Insup Choi
human	WB,IHC	Nat Commun; Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.; Authors - Ludovic Boytard
mouse,human	WB,IF	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB,IF	J Crohns Colitis; Matrix Metalloproteinase MMP-12 promotes macrophage transmigration across intestinal epithelial tight junctions and increases severity of experimental colitis.; Authors - Meghali Nighot
human	WB	Redox Biol; Hydrogen sulfide attenuates cigarette smoke-induced airway remodeling by upregulating SIRT1 signaling pathway.; Authors - Ruijuan Guan
mouse	IHC	Respir Res; Role of PM2.5 in the development and progression of COPD and its mechanisms.; Authors - Junling Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hongxue (Verified Customer) (01-17-2023)

IHC staining in mouse liver, macrophage specific positive signaling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver

MMP12 Antibody Immunohistochemistry validation (1:500 dilution) in Mouse liver (Cat no:22989-1-AP)

FH

Yadavalli (Verified Customer) (07-20-2021)

I had used the MMP12 antibody and detected in the lung tissue which ad shown a significant data

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lung

MMP12 Antibody Western Blot validation (1:1000 dilution) in Lung (Cat no:22989-1-AP)

FH

Hongxue (Verified Customer) (01-12-2021)

wb band very good

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

MMP12 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:22989-1-AP)