MME,CD10 Polyklonaler Antikörper

MME,CD10 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 18008-1-AP

Synonyme

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse kidney using 18008-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 18008-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 18008-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18008-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 18008-1-AP

  IP Result of anti-MME,CD10 (IP:18008-1-AP, 3ug; Detection:18008-1-AP 1:500) with Raji cells lysate 2400ug.

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human kidney using 18008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 18008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Ramos using 18008-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (18008-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, humanes Nierengewebe, Rattennierengewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Nierengewebe
• Erfolgreiche Detektion in FC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

18008-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MME,CD10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MME,CD10 fusion protein Ag12506
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 80aa,9 kDa; 750aa,85 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC106070
• Gene symbol: MME
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Publikationen

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
human	WB	Int J Biol Macromol; A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.; Authors - Hui Zeng
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue
human	IHC	BMC Genomics; Genetic control of renal tumorigenesis by the mouse Rtm1 locus.; Authors - Jensen José Ricardo JR
mouse	WB	J Alzheimers Dis; Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity.; Authors - Ling-Li Lv

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Boyan (Verified Customer) (10-04-2021)

calculated size is 85 kd. This antibody recognized major bands at 60, 30 kd.

• Applications: Western Blot

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C