MKP-2 Monoklonaler Antikörper

MKP-2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1G1C12

Kat-Nr. : 66349-1-Ig

Synonyme

dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2

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Galerie der Validierungsdaten

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  WB analysis using 66349-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66349-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66349-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66349-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 66349-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66349-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66349-1-Ig (MKP-2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66349-1-Ig (MKP-2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66349-1-Ig (MKP-2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66349-1-Ig (MKP-2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66349-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66349-1-Ig(MKP-2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, HepG2-Zellen, Maushirngewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattenhirngewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66349-1-Ig bindet in WB, IHC, IF, CoIP, ELISA MKP-2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MKP-2 fusion protein Ag1206
• Vollständiger Name: dual specificity phosphatase 4
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa, 33 kDa
• GenBank-Zugangsnummer: BC014565
• Gene symbol: DUSP4
• Gene ID (NCBI): 1846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).

Publikationen

Species	Application	Title
rat	IHC	Mol Neurobiol; Dual Specificity Phosphatases Support Axon Plasticity and Viability.; Authors - Ambika Chandrasekhar
human	WB,IHC	J Cancer; PMID: 31258782; Authors - Xianqing Chen
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu
human,mouse	WB,IHC,CoIP	Cell Rep; DUSP4 promotes esophageal squamous cell carcinoma progression by dephosphorylating HSP90β; Authors - Liting Zhou ; KD Validated
human	IHC	Cancers (Basel); Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases; Authors - Maximiliano Gelli
human	IF	Life Sci Alliance; DOCK1 insufficiency disrupts trophoblast function and pregnancy outcomes via DUSP4-ERK pathway; Authors - Yichi Xu