MKL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MKL1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (1)

Kat-Nr. : 21166-1-AP

Synonyme

BSAC, KIAA1438, MAL, MKL/myocardin like protein 1, MKL1, MRTF A, MRTFA, MRTF-A

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Galerie der Validierungsdaten

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  WB analysis using 21166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21166-1-AP (MKL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21166-1-AP (MKL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21166-1-AP

  Immunofluorescent analysis of HeLa cells using 21166-1-AP (MKL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Leberkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 14 publications below
• ChIP: See 4 publications below

Produktinformation

21166-1-AP bindet in WB, IHC, IF, ChIP, ELISA MKL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MKL1 fusion protein Ag15240
• Vollständiger Name: megakaryoblastic leukemia (translocation) 1
• Berechnetes Molekulargewicht: 931 aa, 99 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC115039
• Gene symbol: MKL1
• Gene ID (NCBI): 57591
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Cell Stem Cell; An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling.; Authors - Allison P Kann
mouse	IF	Nat Cell Biol; Myc-dependent dedifferentiation of Gata6+ epidermal cells resembles reversal of terminal differentiation; Authors - Miguel Bernabé-Rubio
	IF	J Cell Biol; The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression.; Authors - Jaime Fernández-Barrera
mouse	IF	Elife; Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors.; Authors - Colin J Dinsmore
human	WB,IHC,IF,ChIP	Exp Mol Med; MRTF-A-NF-κB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-β; Authors - Fu Du ; KD Validated
human	WB	Oncotarget; The Wnt-β-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells.; Authors - Hongpeng He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Qingxue (Verified Customer) (06-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs