MIP-3 Alpha Polyklonaler Antikörper

MIP-3 Alpha Polyklonal Antikörper für IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 26527-1-AP

Synonyme

Beta chemokine exodus 1, C C motif chemokine 20, CC chemokine LARC, CCL20, CKb4, LARC, MIP 3 alpha, MIP 3a, MIP3A, MIP-3α, SCYA20, Small inducible cytokine A20, ST38

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Galerie der Validierungsdaten

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  IHC staining of human lung cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

26527-1-AP bindet in IHC, IF, ELISA MIP-3 Alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIP-3 Alpha fusion protein Ag24069
• Vollständiger Name: chemokine (C-C motif) ligand 20
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC020698
• Gene symbol: CCL20
• Gene ID (NCBI): 6364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

Publikationen

Species	Application	Title
human	IF	Gut Microbes; Fusobacterium nucleatum promotes colorectal cancer metastasis through miR-1322/CCL20 axis and M2 polarization; Authors - Chaochao Xu
human	IF	Front Cell Dev Biol; EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma.; Authors - Bin Qi
mouse		Phytomedicine; The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides.; Authors - Gaofeng Cai
human	IHC	Oncol Rep; DEPDC1 drives hepatocellular carcinoma cell proliferation, invasion and angiogenesis by regulating the CCL20/CCR6 signaling pathway.; Authors - Wubin Guo
human	IHC	Inflammation; PMID: 31838663; Authors - YingYing Hu
human	IHC	Oncol Lett; Overexpression of B7-H4 promotes renal cell carcinoma progression by recruiting tumor-associated neutrophils via upregulation of CXCL8.; Authors - Anqi Li