MICA Monoklonaler Antikörper

MICA Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1E2C8

Kat-Nr. : 66384-1-Ig

Synonyme

MIC A, MICA, PERB11.1

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Galerie der Validierungsdaten

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  WB analysis using 66384-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66384-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66384-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66384-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66384-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 66384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HaCaT-Zellen, A431-Zellen, Daudi-Zellen, HeLa-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

66384-1-Ig bindet in WB, IHC, IF, ELISA MICA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MICA fusion protein Ag3298
• Vollständiger Name: MHC class I polypeptide-related sequence A
• Berechnetes Molekulargewicht: 383 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43-50 kDa
• GenBank-Zugangsnummer: BC016929
• Gene symbol: MICA
• Gene ID (NCBI): 100507436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993, 11491531).

Publikationen

Species	Application	Title
human	WB,IHC,IF	Cancer Lett; Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer; Authors - Deli Mao
human	IF	Pharmacol Res; IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.; Authors - Xin Fang