MHC Class II (I-A/I-E) Monoklonaler Antikörper

MHC Class II (I-A/I-E) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

M5/114.15.2

Kat-Nr. : 65122-1-Ig

Synonyme

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using 65122-1-Ig

  1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse MHC Class II (I-A/I-E) (65122-1-Ig, Clone: M5/114.15.2) and FITC anti-rat IgG2b Antibody at dilution 1:100 (red), or 0.5 ug Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

65122-1-Ig bindet in IF, FC MHC Class II (I-A/I-E) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aktivierte C57BL/6-Mausmilzzellen
• Vollständiger Name: histocompatibility 2, class II antigen A, alpha
• GenBank-Zugangsnummer: BC031711
• Gene symbol: H2-Aa
• Gene ID (NCBI): 14960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Publikationen

Species	Application	Title
mouse	IF	Int Immunopharmacol; Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4+ T cells in chronic liver injury; Authors - Yuwei Zhang