MHC Class II (I-A/I-E) Monoklonaler Antikörper

MHC Class II (I-A/I-E) Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

M5/114.15.2

Kat-Nr. : CL488-65122

Synonyme

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using CL488-65122

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse MHC Class II (I-A/I-E) (CL488-65122, Clone: M5/114.15.2) at dilution of 1:400.

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  FC experiment of mouse splenocytes using CL488-65122

  1X10^6 mouse splenocytes were surface co-stained with CoraLite®647 Anti-Mouse CD3 (17A2) and 0.25 ug CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (CL488-65122, Clone:M5/114.15.2) or 0.25 ug CoraLite® Plus 488-conjugated Rat IgG2b isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65122 bindet in IF, FC MHC Class II (I-A/I-E) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aktivierte C57BL/6-Mausmilzzellen
• Vollständiger Name: histocompatibility 2, class II antigen A, alpha
• GenBank-Zugangsnummer: BC031711
• Gene symbol: H2-Aa
• Gene ID (NCBI): 14960
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).