MGC33407/ACTL9 Polyklonaler Antikörper

MGC33407/ACTL9 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29885-1-AP

Synonyme

Actin like protein 9, ACTL7C, ACTL9, HSD21, hypothetical protein MGC33407

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Galerie der Validierungsdaten

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  WB analysis using 29885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 29885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 29885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 29885-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 29885-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, rat skeletal muscle
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29885-1-AP bindet in WB, IHC, IF, ELISA MGC33407/ACTL9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGC33407/ACTL9 fusion protein Ag31805
• Vollständiger Name: hypothetical protein MGC33407
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC024028
• Gene symbol: MGC33407
• Gene ID (NCBI): 284382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility.