MGAT1 Polyklonaler Antikörper

MGAT1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 15103-1-AP

Synonyme

GGNT1, GlcNAc T I, GLCNAC TI, GLCT1, GLYT1, GNT 1, GNT I, MGAT, MGAT1

---

Galerie der Validierungsdaten

• 

  WB analysis of Y79 using 15103-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15103-1-AP (MGAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15103-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15103-1-AP (MGAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15103-1-AP (MGAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse liver using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse liver using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15103-1-AP

  Immunofluorescent analysis of Hela cells, using MGAT1 antibody 15103-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe, Mausherzgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15103-1-AP bindet in WB, IHC, IF, ELISA MGAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGAT1 fusion protein Ag7446
• Vollständiger Name: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 51-55 kDa
• GenBank-Zugangsnummer: BC003575
• Gene symbol: MGAT1
• Gene ID (NCBI): 4245
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Arthritis Rheum; Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage.; Authors - Urita Atsushi A
mouse	WB	FASEB J; Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.; Authors - Andrew R Ednie
human, rat	WB	Schizophr Res; Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia.; Authors - Jordyn M Kippe
mouse	WB	Liver Transpl; Monoacylglycerol Acyltransferase 1 Knockdown Exacerbates Hepatic Ischemia/Reperfusion Injury in Mice With Hepatic Steatosis.; Authors - Kim H H Liss
rat	WB,IF	Sci Rep; Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats.; Authors - Kaimei Lu
human	WB	Biochemistry; N-linked glycosylation dependent and independent mechanisms regulating CTRP12 cleavage, secretion, and stability.; Authors - Ashley N Stewart