MFN1 Monoklonaler Antikörper

MFN1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3F11C11

Kat-Nr. : CL594-66776

Synonyme

DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL594-66776

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated MFN1 antibody (CL594-66776, Clone: 3F11C11 ) at dilution of 1:100.

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  IF Staining of T-47D using CL594-66776

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using CoraLite®594-conjugated MFN1 antibody (CL594-66776, Clone: 3F11C11 ) at dilution of 1:100.

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  FC experiment of HepG2 using CL594-66776

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MFN1 (CL594-66776, Clone:3F11C11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66776 bindet in IF, FC (Intra) MFN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MFN1 fusion protein Ag4890
• Vollständiger Name: mitofusin 1
• Berechnetes Molekulargewicht: 741 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC040557
• Gene symbol: MFN1
• Gene ID (NCBI): 55669
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C