MFG-E8 Monoklonaler Antikörper

MFG-E8 Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1A9

Kat-Nr. : 67797-1-Ig

Synonyme

BA46, Breast epithelial antigen BA46, EDIL1, HMFG, hP47, HsT19888, Lactadherin, MFG E8, MFGE8, MFG-E8, MFGM, Milk fat globule EGF factor 8, OAcGD3S, SED1, SPAG10

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Galerie der Validierungsdaten

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  WB analysis of human milk using 67797-1-Ig

  human milk were subjected to SDS PAGE followed by western blot with 67797-1-Ig (MFG-E8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67797-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67797-1-Ig (MFG-E8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 67797-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67797-1-Ig (MFG-E8 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67797-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67797-1-Ig (MFG-E8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MDA-MB-231 using 67797-1-Ig

  1X10^6 MDA-MB-231 cells were stained with 0.2 ug Anti-Human MFG-E8 (67797-1-Ig, Clone: 1A1A9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human milk, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67797-1-Ig bindet in WB, IHC, FC, ELISA MFG-E8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MFG-E8 fusion protein Ag30666
• Vollständiger Name: milk fat globule-EGF factor 8 protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC003610
• Gene symbol: MFGE8
• Gene ID (NCBI): 4240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearance of apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity and cancer metastasis (PMID: 23972256). MFG-E8 consists of two-repeated EGF-likedomains, a mucin-like domain and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain (PMID: 11856354). It shows ubiquitous pattern of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with a number of diseases (PMID: 27429513).