MFF Monoklonaler Antikörper

MFF Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1G4B12

Rezensionen (1)

Kat-Nr. : 66527-1-Ig

Synonyme

AD033, C2orf33, MFF, mitochondrial fission factor

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Galerie der Validierungsdaten

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  WB analysis of pig brain using 66527-1-Ig

  pig/rat/mouse brain tissues were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66527-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66527-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66527-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66527-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66527-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFF antibody (66527-1-Ig, Clone: 1G4B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, HepG2-Zellen, L02-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66527-1-Ig bindet in WB, IHC, IF, ELISA MFF und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MFF fusion protein Ag9990
• Vollständiger Name: mitochondrial fission factor
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC000797
• Gene symbol: MFF
• Gene ID (NCBI): 56947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Publikationen

Species	Application	Title
rat	WB	Phytother Res; Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow; Authors - Jing-Jing Li
mouse	WB	J Adv Res; Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics; Authors - Liuyang Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

JAE HO (Verified Customer) (05-06-2019)

25 ug of PC3 cell lysates were used for western blot.500 ug of cell lysates and 2.5 ug of antibodies were used for IP.Comparing to "17090-1-AP", it looks less titer but it works well for WB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PC3 cell