MFAP5 Polyklonaler Antikörper

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• KD/KO Validated

MFAP5 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15727-1-AP

Synonyme

MAGP 2, MAGP2, MFAP 5, MFAP5, MP25

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15727-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15727-1-AP (MFAP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 15727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15727-1-AP (MFAP5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15727-1-AP (MFAP5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15727-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15727-1-AP (MFAP5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15727-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15727-1-AP (MFAP5 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 5 publications below

Produktinformation

15727-1-AP bindet in WB, IHC, ELISA MFAP5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MFAP5 fusion protein Ag8467
• Vollständiger Name: microfibrillar associated protein 5
• Berechnetes Molekulargewicht: 173 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC005901
• Gene symbol: MFAP5
• Gene ID (NCBI): 8076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, IHC	Hepatology; YAP promotes proliferation, chemoresistance and angiogenesis in human cholangiocarcinoma via TEAD transcription factors.; Authors - Patricia Marti ; KD Validated
human	IHC	Clin Transl Med; Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.; Authors - Qian Hao
human	IHC	Front Oncol; DPP6 and MFAP5 are associated with immune infiltration as diagnostic biomarkers in distinguishing uterine leiomyosarcoma from leiomyoma; Authors - Yumin Ke
human	IHC	Hum Cell; Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer.; Authors - Zhao Zhang
human	WB	Clin Transl Oncol; Stromal fibroblast-derived MFAP5 promotes the invasion and migration of breast cancer cells via Notch1/slug signaling.; Authors - Z Chen ; KD Validated
human	IHC	Int J Mol Sci; Microfibril Associated Protein 5 (MFAP5) Is Related to Survival of Ovarian Cancer Patients but Not Useful as a Prognostic Biomarker; Authors - Katarzyna Aleksandra Kujawa