METTL3 Monoklonaler Antikörper

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• KD/KO Validated

METTL3 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4B4A3

Kat-Nr. : 67733-2-Ig

Synonyme

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

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Galerie der Validierungsdaten

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  WB analysis using 67733-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67733-2-Ig (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67733-2-Ig

  WB result of METTL3 antibody (67733-2-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected HeLa cells.

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  IHC staining of human lung cancer using 67733-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 67733-2-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 67733-2-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67733-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67733-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67733-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Urothelkarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67733-2-Ig bindet in WB, IHC, ELISA METTL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: METTL3 fusion protein Ag7110
• Vollständiger Name: methyltransferase like 3
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC001650
• Gene symbol: METTL3
• Gene ID (NCBI): 56339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.