METTL16 Polyklonaler Antikörper

METTL16 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 19924-1-AP

Synonyme

METT10D, METTL16

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Galerie der Validierungsdaten

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  WB analysis using 19924-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19924-1-AP (METTL16 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 19924-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 19924-1-AP (METTL16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19924-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19924-1-AP (METT10D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 19924-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19924-1-AP (METT10D antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 19924-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19924-1-AP (METTL16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 19924-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19924-1-AP (METTL16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below

Produktinformation

19924-1-AP bindet in WB, IHC, ELISA METTL16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METTL16 fusion protein Ag13889
• Vollständiger Name: methyltransferase 10 domain containing
• Berechnetes Molekulargewicht: 562 aa, 64 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC001213
• Gene symbol: METT10D
• Gene ID (NCBI): 79066
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methyltransferase-like protein 16 (METTL16) is a human RNA methyltransferase that installs m6A marks on U6 small nuclear RNA (U6 snRNA) and S-adenosylmethionine (SAM) synthetase pre-mRNA. METTL16 is considered a protective gene, suppressing the development of OC, HCC, and endocrine system tumors (PMID: 33237039, PMID: 33133142, PMID: 34187307). However, the high expression of METTL16 has been linked with a poor survival rate in patients with breast cancer (PMID: 33362863). METTL16 has 2 isoforms with molecular weights of 64 and 26 kDa.

Publikationen

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	Oxid Med Cell Longev; Methylation Pattern Mediated by m6A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma.; Authors - Feng Jiang
human	WB	J Biol Chem; m6A demethylation of cytidine deaminase APOBEC3B mRNA orchestrates arsenic-induced mutagenesis.; Authors - Ming Gao
human	WB,IHC	Anal Cell Pathol (Amst); METTL16 Inhibits the Malignant Progression of Epithelial Ovarian Cancer through the lncRNA MALAT1/β-Catenin Axis; Authors - Changshu Li ; KD Validated
human	IHC	J Coll Physicians Surg Pak; Clinical Significance of Methyltransferase-like 16 Expression in Epithelial Ovarian Cancer; Authors - Changshu Li