METAP1 Polyklonaler Antikörper

METAP1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28702-1-AP

Synonyme

DKFZp781C0419, KIAA0094, MAP 1, MetAP 1, METAP1, Methionine aminopeptidase 1, methionyl aminopeptidase 1, Peptidase M 1

---

Galerie der Validierungsdaten

• 

  WB analysis using 28702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28702-1-AP (METAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28702-1-AP (METAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, HeLa-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28702-1-AP bindet in WB, ELISA METAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METAP1 fusion protein Ag29828
• Vollständiger Name: methionyl aminopeptidase 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: NM_015143
• Gene symbol: METAP1
• Gene ID (NCBI): 23173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METAP1 (methionyl aminopeptidase 1), also known as MAP1A or MetAP1A, is expected to be located in cytosol and widely expressed in esophagus and testis. The calculated molecular weight of METAP1 is 43 kDa. METAP1 is expected to have aminopeptidase activity and metallo-exopeptidase activity. It is also involved in platelet aggregation. At the same time, HsMetAP1 participates in co-translation to remove N- terminal methionine from newborn protein. Studies have shown that when HsMetAP1 is knocked down by gene-specific siRNA, cells show slow progress in G(2)/M phase, which is similar to cells treated with MetAP1 inhibitors. Importantly, MetAP1 inhibitors can induce apoptosis of leukemia cell lines, which may be the result of their interference with G(2)/M checkpoint. Therefore, it is necessary for normal cell cycle, especially in G(2)/M phase of cell cycle, and it can be used as a promising target for discovering and developing new anticancer drugs (PMID: 17114291).