MEPE Polyklonaler Antikörper

MEPE Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23256-1-AP

Synonyme

MEPE, OF45, Osteoblast/osteocyte factor 45

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 23256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 23256-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23256-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23256-1-AP

  IP Result of anti-MEPE (IP:23256-1-AP, 4ug; Detection:23256-1-AP 1:1000) with HeLa cells lysate 4000ug.

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  IHC staining of human lung using 23256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 23256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 23256-1-AP

  Immunofluorescent analysis of HepG2 cells, using MEPE antibody 23256-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23256-1-AP bindet in WB, IP, IHC, IF,ELISA MEPE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEPE fusion protein Ag19758
• Vollständiger Name: matrix extracellular phosphoglycoprotein
• Berechnetes Molekulargewicht: 525 aa, 58 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC128158
• Gene symbol: MEPE
• Gene ID (NCBI): 56955
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a full-length human MEPE protein detects a major band of 98 kDa.