MEPCE Polyklonaler Antikörper

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• KD/KO Validated

MEPCE Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14917-1-AP

Synonyme

Bin3 homolog, MEPCE, methylphosphate capping enzyme

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 14917-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14917-1-AP (MEPCE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14917-1-AP

  WB result of MEPCE antibody (14917-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPCE transfected MCF-7 cells.

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  IP experiment of MCF-7 using 14917-1-AP

  IP Result of anti-MEPCE (IP:14917-1-AP, 4ug; Detection:14917-1-AP 1:400) with MCF-7 cells lysate 3200ug.

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  IF Staining of MCF-7 using 14917-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14917-1-AP (MEPCE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IP: See 1 publications below

Produktinformation

14917-1-AP bindet in WB, IP, IF, ELISA MEPCE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEPCE fusion protein Ag6733
• Vollständiger Name: methylphosphate capping enzyme
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC000556
• Gene symbol: MEPCE
• Gene ID (NCBI): 56257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEPCE is a somponent of the 7SK snRNP complex, and a S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, which leads to its stabilization.

Publikationen

Species	Application	Title
human	WB	Sci Adv; 7SK methylation by METTL3 promotes transcriptional activity; Authors - Marcelo Perez-Pepe
	IP	Nat Commun; Interaction of 7SK with the Smn complex modulates snRNP production.; Authors - Changhe Ji
human	WB	EMBO Rep; Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.; Authors - Ahmed S Warda
human	WB	MBio; Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.; Authors - Juanita Von Dwingelo
human	WB	Vaccines (Basel); HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation.; Authors - Nur Firdaus Isa
human	WB	Sci Rep; de novo MEPCE nonsense variant associated with a neurodevelopmental disorder causes disintegration of 7SK snRNP and enhanced RNA polymerase II activation.; Authors - Pauline E Schneeberger