MEF2C Polyklonaler Antikörper

MEF2C Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18290-1-AP

Synonyme

MEF2C, myocyte enhancer factor 2C

---

Galerie der Validierungsdaten

• 

  WB analysis using 18290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18290-1-AP (MEF2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 18290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (18290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 18290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (18290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

18290-1-AP bindet in WB, IHC, IF, ChIP, ELISA MEF2C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myocyte enhancer factor 2C
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 45-70 kDa
• GenBank-Zugangsnummer: NM_002397
• Gene symbol: MEF2C
• Gene ID (NCBI): 4208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

Publikationen

Species	Application	Title
		Nature; Genetic and epigenetic coordination of cortical interneuron development.; Authors - Kathryn C Allaway ; KO Validated
human	ChIP	Nature; Evolution of Osteocrin as an activity-regulated factor in the primate brain.; Authors - Bulent Ataman
human	IHC	Biol Reprod; Integrated microRNA and mRNA network analysis of the human myometrial transcriptome in the transition from quiescence to labor.; Authors - William E Ackerman
mouse	IF	Cell Rep; Chromatin Environment and Cellular Context Specify Compensatory Activity of Paralogous MEF2 Transcription Factors.; Authors - Shahriyar P Majidi
human	ChIP	Cell Genom; Single nucleus multiomics identifies ZEB1 and MAFB as candidate regulators of Alzheimer's disease-specific cis-regulatory elements; Authors - Ashlyn G Anderson