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MED23 Polyklonaler Antikörper

MED23 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27850-1-AP

Synonyme

ARC130, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DRIP130, hSur 2, KIAA1216, MED23, mediator complex subunit 23, Protein sur 2 homolog, SUR2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of A375 cells using MED23 Polyclonal antibody (27850-1-AP)

    WB analysis of A375 using 27850-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 27850-1-AP (MED23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA375-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

27850-1-AP bindet in WB, ELISA MED23 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MED23 fusion protein Ag27241
Vollständiger Name mediator complex subunit 23
Berechnetes Molekulargewicht 156 kDa
Beobachtetes Molekulargewicht 140 kDa, 50 kDa
GenBank-ZugangsnummerBC060759
Gene symbol MED23
Gene ID (NCBI) 9439
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.

Protokolle

Produktspezifische Protokolle
WB protocol for MED23 antibody 27850-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Rep

DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.

Authors - Xu Wang
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

SITING (Verified Customer) (04-02-2021)

not very good， too many unspecific band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
MED23 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:27850-1-AP)