MED12 Polyklonaler Antikörper

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• KD/KO Validated

MED12 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 20028-1-AP

Synonyme

ARC240, CAG repeat protein 45, CAGH45, FGS1, HOPA, KIAA0192, MED12, mediator complex subunit 12, OKS, OPA containing protein, OPA1, TNRC11, TRAP230

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 20028-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20028-1-AP (MED12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20028-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20028-1-AP (MED12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20028-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 20028-1-AP (MED12 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

20028-1-AP bindet in WB, IHC, IF, ELISA MED12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mediator complex subunit 12
• Berechnetes Molekulargewicht: 243 kDa
• Beobachtetes Molekulargewicht: 243 kDa
• GenBank-Zugangsnummer: NM_005120
• Gene symbol: MED12
• Gene ID (NCBI): 9968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.

Publikationen

Species	Application	Title
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
human	IHC,IF	Mod Pathol; MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma.; Authors - Elizabeth Bertsch
human,mouse	WB,IHC,IF	Genes (Basel); MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection.; Authors - Yingchao Zhou ; KD Validated
human	IHC	Pathol Res Pract; Cyclin-dependent kinase 8 is an independent prognosticator in uterine leiomyosarcoma.; Authors - Nobuko Yasutake