MDC1 Polyklonaler Antikörper

MDC1 Polyklonal Antikörper für IF, IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 24721-1-AP

Synonyme

KIAA0170, MDC1, NFBD1

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Galerie der Validierungsdaten

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  IHC staining of human testis using 24721-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24721-1-AP (MDC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 24721-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24721-1-AP (MDC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24721-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24721-1-AP (MDC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human appendix using 24721-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 24721-1-AP (MDC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human appendix using 24721-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 24721-1-AP (MDC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 24721-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24721-1-AP (MDC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HEK-293 using 24721-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24721-1-AP (MDC1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HeLa using 24721-1-AP

  Immunofluorescent analysis of HeLa cells using 24721-1-AP (MDC1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

24721-1-AP bindet in WB, IHC, IF, ELISA MDC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MDC1 fusion protein Ag20135
• Vollständiger Name: mediator of DNA damage checkpoint 1
• Berechnetes Molekulargewicht: 2089 aa, 227 kDa
• GenBank-Zugangsnummer: BC152556
• Gene symbol: MDC1
• Gene ID (NCBI): 9656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

Publikationen

Species	Application	Title
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao
mouse	IF	Cell Death Dis; Wnt signaling induces radioresistance through upregulating HMGB1 in esophageal squamous cell carcinoma.; Authors - Yuanyuan Zhao
human	IF	Cancers (Basel); SFN Enhanced the Radiosensitivity of Cervical Cancer Cells via Activating LATS2 and Blocking Rad51/MDC1 Recruitment to DNA Damage Site.; Authors - Shiyu Wang
human	WB	DNA Repair (Amst); Functional defects of cancer-associated MDC1 mutations in DNA damage repair; Authors - Rong Xie