MCT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MCT1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

57

Rezensionen (3)

Kat-Nr. : 20139-1-AP

Synonyme

HHF7, MCT, MCT 1, MCT1, Monocarboxylate transporter 1, SLC16A1

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Galerie der Validierungsdaten

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  WB analysis using 20139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20139-1-AP

  WB result of MCT1 antibody (20139-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT1 transfected HeLa cells.

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  WB analysis of mouse brain using 20139-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20139-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 20139-1-AP

  IP result of anti-MCT1 (IP:20139-1-AP, 4ug; Detection:20139-1-AP 1:300) with Raji cells lysate 3600 ug.

• 

  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20139-1-AP (MCT1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MCT1 antibody (20139-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MCT1 antibody (20139-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslebergewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 41 publications below
• IHC: See 17 publications below
• IF: See 8 publications below
• FC: See 1 publications below

Produktinformation

20139-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MCT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCT1 fusion protein Ag14098
• Vollständiger Name: solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
• Berechnetes Molekulargewicht: 500 aa, 54 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC026317
• Gene symbol: SLC16A1
• Gene ID (NCBI): 6566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.

Publikationen

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Nat Cancer; Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer; Authors - Federica Cappellesso
mouse	IHC	Clin Cancer Res; A Novel Role for DNA-PK in Metabolism by Regulating Glycolysis in Castration Resistant Prostate Cancer.; Authors - Emanuela Dylgjeri
human	WB,IHC	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang
rat	WB	Acta Pharmacol Sin; Pinocembrin attenuates hemorrhagic transformation after delayed t-PA treatment in thromboembolic stroke rats by regulating endogenous metabolites.; Authors - Ling-Lei Kong
human	WB,IHC	Haematologica; Inhibition of monocarboxylate transporter 1 by AZD3965 as a novel therapeutic approach for the treatment of diffuse large B-cell lymphoma and Burkitt lymphoma.; Authors - Richard A Noble

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xie (Verified Customer) (07-07-2023)

The expression of MCT4 was seen in my samples.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: brain

MCT1 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:20139-1-AP)

FH

Aderonke (Verified Customer) (05-23-2023)

The expression of MCT4 was seen in three of the five cell lines used without an inhibitor. Also, an inhibtor of MCT4, was used at two different concentrations and the expression was decreased in the cells that expressed the protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: Head and Neck cancer cell lines

FH

Huseyin (Verified Customer) (07-28-2022)

I used the antibody with 1:3000 dilution in TBS-T solution O/N and I received correct size bands in Ishikawa cell line.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Ishikawa Cell Line