MCRS1 Monoklonaler Antikörper

MCRS1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H10E10

Kat-Nr. : 60215-1-Ig

Synonyme

58 kDa microspherule protein, ICP22BP, INO80 complex subunit J, INO80Q, MCRS1, MCRS2, microspherule protein 1, MSP58, P78

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Galerie der Validierungsdaten

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  WB analysis of ROS1728 using 60215-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60215-1-Ig (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 60215-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 60215-1-Ig (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ROS1728-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60215-1-Ig bindet in WB, ELISA MCRS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MCRS1 fusion protein Ag1904
• Vollständiger Name: microspherule protein 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55+110 kDa
• GenBank-Zugangsnummer: BC011794
• Gene symbol: MCRS1
• Gene ID (NCBI): 10445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCRS1, also named INO80Q, MSP58 or p78, is a nucleolar protein expressed in the very early S phase. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 is also present on polyribosomes of synaptoneurosome as a novel RNA-binding protein to activate the rRNA transcription. The forkhead-associated domain of MCRS1 is involved in association with centrosomal protein Nde1, comprising a component of the centrosome and taking an essential role in viability. Its isoform MCRS2 might be a linker between telomere maintenance and cell-cycle regulation by interacting with LPTS/PinX1, a telomerase-inhibitory protein. MCRS1 is detected by Western blot as a highly modified protein at 78 kDa and minor bands at about 62 and 55 kDa.