MCM7 Monoklonaler Antikörper

MCM7 Monoklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G1F6

Kat-Nr. : 67446-1-Ig

Synonyme

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

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Galerie der Validierungsdaten

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  WB analysis using 67446-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67446-1-Ig (MCM7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 67446-1-Ig

  IP result of anti-MCM7(IP:67446-1-Ig, 4ug; Detection:67446-1-Ig 1:2000) with HeLa cells lysate 1040 ug.

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  IHC staining of human breast cancer using 67446-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67446-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67446-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67446-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, 4T1-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67446-1-Ig bindet in WB, IP, IHC, ELISA MCM7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MCM7 fusion protein Ag28558
• Vollständiger Name: minichromosome maintenance complex component 7
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC013375
• Gene symbol: MCM7
• Gene ID (NCBI): 4176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2–7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].