MAVS; VISA Rekombinanter Antikörper

MAVS; VISA Rekombinant Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

7F14

Kat-Nr. : 81910-1-RR

Synonyme

CARDIF, DKFZp547C224, DKFZp666M015, FLJ27482, FLJ35386, FLJ38051, FLJ41962, IPS 1, IPS1, KIAA1271, MAVS, VISA

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Galerie der Validierungsdaten

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  WB analysis using 81910-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81910-1-RR (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 81910-1-RR

  IP result of anti-MAVS; VISA(IP:81910-1-RR, 4ug; Detection:81910-1-RR 1:4000) with HEK-293 cells lysate 1480 ug.

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  IHC staining of human breast cancer using 81910-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human breast cancer using 81910-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HeLa using 81910-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 81910-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81910-1-RR bindet in WB, IP, IHC, IF, ELISA MAVS; VISA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAVS; VISA fusion protein Ag5655
• Vollständiger Name: mitochondrial antiviral signaling protein
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC044952
• Gene symbol: MAVS
• Gene ID (NCBI): 57506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.