MAVS; VISA Monoklonaler Antikörper

MAVS; VISA Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1A8E9

Kat-Nr. : 66911-1-Ig

Synonyme

CARDIF, DKFZp547C224, DKFZp666M015, FLJ27482, FLJ35386, FLJ38051, FLJ41962, IPS 1, IPS1, KIAA1271, MAVS, MAVS; VISA, VISA

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Galerie der Validierungsdaten

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  WB analysis of A431 using 66911-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66911-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66911-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:550-1:2200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below

Produktinformation

66911-1-Ig bindet in WB, IHC, IF, ELISA MAVS; VISA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAVS; VISA fusion protein Ag5949
• Vollständiger Name: mitochondrial antiviral signaling protein
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 70-75 kDa
• GenBank-Zugangsnummer: BC044952
• Gene symbol: MAVS
• Gene ID (NCBI): 57506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Publikationen

Species	Application	Title
	IF	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human,mouse	WB	J Immunother Cancer; Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer.; Authors - Xiaohua Jie
human	WB	Front Immunol; Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.; Authors - Zhihai Zhou
human	WB	J Virol; Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2.; Authors - Yumei Han
human	WB	Vet Microbiol; DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation; Authors - Shujuan Xu
human	WB,IF	Virus Res; Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro.; Authors - Qian Li