Matrilin 2 Polyklonaler Antikörper

Matrilin 2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 24064-1-AP

Synonyme

MATN2, Matrilin 2

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 24064-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24064-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24064-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293-Zellen, Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

24064-1-AP bindet in WB, IF, ELISA Matrilin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Matrilin 2 fusion protein Ag21113
• Vollständiger Name: matrilin 2
• Berechnetes Molekulargewicht: 107 kDa
• Beobachtetes Molekulargewicht: 120-150 kDa
• GenBank-Zugangsnummer: BC010444
• Gene symbol: MATN2
• Gene ID (NCBI): 4147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The matrilins represent a new family of oligomeric non-collagenous extracellular matrix proteins that are assumed to act as adapter molecules connecting other proteins and proteoglycans in the extracellular matrix (PMID: 17513098). Four matrilins (matrilin-1, -2, -3, and -4) have been identified. They all share a structure made up of von Willebrand factor A domains, EGF-like domains and a coiled-coil alpha-helical module (PMID: 18386166). Matrilin-1 and matrilin-3 are expressed mainly in cartilage, while matrilin-2 and matrilin-4 show broader tissue distribution. Matrilin-2 is the largest member of the family. It incorporates into loose and dense connective tissue and becomes associated with some basement membranes (PMID: 17513098).

Publikationen

Species	Application	Title
zebrafish	WB	Sci Adv; A basement membrane discovery pipeline uncovers network complexity, regulators, and human disease associations.; Authors - Ranjay Jayadev ; KD Validated
mouse	WB,IF	J Mol Med (Berl); Valproic acid modulates collagen architecture in the postoperative conjunctival scar.; Authors - Li-Fong Seet
human	WB	Int J Mol Sci; A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions; Authors - Ilenia Matera