MAT2B Polyklonaler Antikörper

MAT2B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 15952-1-AP

Synonyme

MAT II, MAT II beta, MAT2B, MATIIbeta, Nbla02999, SDR23E1, TGR, UNQ2435/PRO4995

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15952-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15952-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15952-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15952-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15952-1-AP

  IP Result of anti-MAT2B (IP:15952-1-AP, 4ug; Detection:15952-1-AP 1:500) with HepG2 cells lysate 1000ug.

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  IHC staining of human lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15952-1-AP (MAT2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15952-1-AP (MAT2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15952-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAT2B antibody 15952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 15952-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAT2B antibody 15952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen, Maushirngewebe, Maushodengewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Nierengewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 1 publications below

Produktinformation

15952-1-AP bindet in WB, IP, IHC, IF, ELISA MAT2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAT2B fusion protein Ag8649
• Vollständiger Name: methionine adenosyltransferase II, beta
• Berechnetes Molekulargewicht: 36 kDa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC005218
• Gene symbol: MAT2B
• Gene ID (NCBI): 27430
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human		Ann N Y Acad Sci; PMID: 29722030; Authors - Holly M Brown-Borg
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia
chicken	WB	Sci Rep; In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications.; Authors - Yun Hu
rat	WB	J Nutr Biochem; Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats.; Authors - Nannan Zhao