MAST3 Polyklonaler Antikörper

MAST3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19507-1-AP

Synonyme

KIAA0561, MAST3

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 19507-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19507-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19507-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19507-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19507-1-AP

  IP Result of anti-MAST3 (IP:19507-1-AP, 4ug; Detection:19507-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human kidney using 19507-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19507-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 19507-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAST3 antibody 19507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hirngewebe, Maushirngewebe, Mauslungengewebe, Rattenhirngewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19507-1-AP bindet in WB, IP, IHC, IF, ELISA MAST3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: microtubule associated serine/threonine kinase 3
• Berechnetes Molekulargewicht: 143 kDa
• Beobachtetes Molekulargewicht: 144 kDa
• GenBank-Zugangsnummer: NM_015016
• Gene symbol: MAST3
• Gene ID (NCBI): 23031
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAST3, also named as KIAA0561, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. MAST3 catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.