MAS1L-Specific Polyklonaler Antikörper

MAS1L-Specific Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 20080-1-AP

Synonyme

MAS L, MAS R, MAS1 like, MAS1 oncogene like, MAS1L, MRG

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 20080-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20080-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20080-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20080-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20080-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20080-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAS1L antibody 20080-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

20080-1-AP bindet in WB, IHC, IF, ELISA MAS1L-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: MAS1 oncogene-like
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_052967
• Gene symbol: MAS1L
• Gene ID (NCBI): 116511
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAS1L, also named as MRG, belongs to the G-protein coupled receptor 1 family and Mas subfamily. The antibody is specific to MAS1L.

Publikationen

Species	Application	Title
mouse	WB	J Transl Med; ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway; Authors - Feng Chen
mouse	WB	J Inflamm Res; The ACE2-Ang-(1‑7)-Mas Axis Modulates M1/M2 Macrophage Polarization to Relieve CLP-Induced Inflammation via TLR4-Mediated NF-кb and MAPK Pathways.; Authors - Hang Pan
mouse	IHC,WB	ACS Omega; Azelaic Acid Regulates the Renin-Angiotensin System and Improves Colitis Based on Network Pharmacology and Experimentation; Authors - Yangjie Liao
rat	WB	Neural Plast; Electroacupuncture Improved Chronic Cerebral Hypoperfusion-Induced Anxiety-Like Behavior and Memory Impairments in Spontaneously Hypertensive Rats by Downregulating the ACE/Ang II/AT1R Axis and Upregulating the ACE2/Ang-(1-7)/MasR Axis.; Authors - Peipei Feng
rat	WB,IHC	Pharmacology; Canagliflozin Ameliorates Ventricular Remodeling through Apelin/Angiotensin-Converting Enzyme 2 Signaling in Heart Failure with Preserved Ejection Fraction Rats; Authors - Tingting Zhang
mouse	WB	Arch Biochem Biophys; ACE2 activation alleviates sepsis-induced cardiomyopathy by promoting MasR-Sirt1-mediated mitochondrial biogenesis; Authors - Tian-Tian Wan