MARK4 Polyklonaler Antikörper

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• KD/KO Validated

MARK4 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 20174-1-AP

Synonyme

KIAA1860, MARK4, MARKL1, Nbla00650

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Galerie der Validierungsdaten

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  IHC staining of human kidney using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20174-1-AP (MARK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20174-1-AP (MARK4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:50.

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  IHC staining of human colon using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:50.

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  IHC staining of human gliomas using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Gliomgewebe, humanes Hodengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

20174-1-AP bindet in IP, IHC, IF, ELISA MARK4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARK4 fusion protein Ag14013
• Vollständiger Name: MAP/microtubule affinity-regulating kinase 4
• Berechnetes Molekulargewicht: 752 aa, 83 kDa
• GenBank-Zugangsnummer: BC009049
• Gene symbol: MARK4
• Gene ID (NCBI): 57787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARK4(MAP/microtubule affinity-regulating kinase 4) is also named as KIAA1860, MARKL1 and belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. It is directly involved in microtubule organization in neuronal cells and may contribute to the pathological phosphorylation of tau in Alzheimer's disease and it also plays a role in hepatocellular carcinogenesis. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 83 kDa and 75 kDa. MARK4 can exsit as 40-65 kDa forms in hippocampal homogenates from Alzheimer`s disease and non-demented elderly cases besides the full-length isoform 84 kDa. (PMID:24533944).

Publikationen

Species	Application	Title
rat		FASEB J; Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization.; Authors - Wenhui Su
mouse	IF	FASEB J; NC1-peptide regulates spermatogenesis through changes in cytoskeletal organization mediated by EB1.; Authors - Shiwen Liu
rat		FASEB J; Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.; Authors - Ying Gao
rat	IF	Endocrinology; Sperm Release at Spermiation Is Regulated by Changes in the Organization of Actin- and Microtubule-Based Cytoskeletons at the Apical Ectoplasmic Specialization-A Study Using the Adjudin Model.; Authors - Linxi Li
rat		Toxicol Sci; Regulation of BTB dynamics in spermatogenesis - insights from the adjudin toxicant model.; Authors - Bai-Ping Mao
human,mouse	IHC	J Oral Microbiol; Microtubule affinity regulating kinase 4 promoted activation of the NLRP3 inflammasome-mediated pyroptosis in periodontitis.; Authors - Lulu Wang