MARK1 Polyklonaler Antikörper

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• KD/KO Validated

MARK1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 21552-1-AP

Synonyme

KIAA1477, MARK, MARK1

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Galerie der Validierungsdaten

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  WB analysis using 21552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21552-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21552-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21552-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 21552-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21552-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21552-1-AP

  IP Result of anti-MARK1 (IP:21552-1-AP, 4ug; Detection:21552-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of rat testis using 21552-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21552-1-AP (MARK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 21552-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21552-1-AP (MARK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Mausnierengewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IP: See 2 publications below

Produktinformation

21552-1-AP bindet in WB, IP, IHC, ELISA MARK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARK1 fusion protein Ag16225
• Vollständiger Name: MAP/microtubule affinity-regulating kinase 1
• Berechnetes Molekulargewicht: 795 aa, 89 kDa
• Beobachtetes Molekulargewicht: 85-89 kDa, 72 kDa
• GenBank-Zugangsnummer: BC113869
• Gene symbol: MARK1
• Gene ID (NCBI): 4139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARK1, also named as MAP-microtubule affinity regulating kinase 1, which form a subfamily of the calcium/calmodulin-dependent protein kinase (CAMP) group of kinases(PMID: 22670221).It is involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1 also act as a regulator of the neuronal migration and Wnt signaling pathway.It has 3 isoforms which molecular weight is 89,84,72 kDa. This antibody may detect all of the isoforms.

Publikationen

Species	Application	Title
human	WB	Mol Cell; An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.; Authors - Jonathan M Goodwin ; KD Validated
human	IP	Nat Commun; TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.; Authors - Tsz-Yin Chan ; KD Validated
mouse	WB	Cell Rep; DIXDC1 Phosphorylation and Control of Dendritic Morphology Are Impaired by Rare Genetic Variants.; Authors - Vickie Kwan
human	WB	Cell Commun Signal; Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.; Authors - Abiola Abdulrahman Ayanlaja
rat	WB,IP	J Biol Chem; Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.; Authors - Qian Wu
rat	WB	PLoS One; MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.; Authors - Laura P Bernard

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Manish (Verified Customer) (06-09-2020)

The antibody works really well. We dilute it 10 fold and still get strong bands on western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000