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MARCKS Polyklonaler Antikörper

MARCKS Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-20661

Synonyme

80K L, 80K L protein, FLJ14368, FLJ90045, MACS, MARCKS, PKCSL, PRKCSL

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®555-conjugated MARCKS Polyclonal antibody (CL555-20661)

    IF Staining of mouse brain using CL555-20661

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®555 MARCKS antibody (CL555-20661) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated MARCKS Polyclonal antibody (CL555-20661)

    FC experiment of HeLa using CL555-20661

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human MARCKS (CL555-20661) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaushirngewebe
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-20661 bindet in IF, FC (Intra) MARCKS und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name myristoylated alanine-rich protein kinase C substrate
Berechnetes Molekulargewicht 32 kDa
Beobachtetes Molekulargewicht 80 kDa
GenBank-ZugangsnummerNM_002356
Gene symbol MARCKS
Gene ID (NCBI) 4082
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Protokolle

Produktspezifische Protokolle
IF protocol for CL555 MARCKS antibody CL555-20661Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen