EB3 Polyklonaler Antikörper

EB3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 23974-1-AP

Synonyme

EB3, End binding protein 3, MAPRE3

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23974-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23974-1-AP (EB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 23974-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EB3 antibody (23974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

23974-1-AP bindet in WB, IHC, IF, ELISA EB3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EB3 fusion protein Ag21148
• Vollständiger Name: microtubule-associated protein, RP/EB family, member 3
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 30-36 kDa
• GenBank-Zugangsnummer: BC011557
• Gene symbol: MAPRE3
• Gene ID (NCBI): 22924
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
mouse	WB	Cell Tissue Res; Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress; Authors - Valentina Grande