JNK Polyklonaler Antikörper

JNK Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 51153-1-AP

Synonyme

c Jun N terminal kinase 2, JNK 55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, MAP kinase 9, MAPK 9, MAPK9, p54a, p54aSAPK, PRKM9, SAPK

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 51153-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 51153-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 51153-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 51153-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 51153-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 51153-1-AP

  IP Result of anti-JNK (IP:51153-1-AP, 4ug; Detection:51153-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  IF Staining of HeLa using 51153-1-AP

  Immunofluorescent analysis of HeLa cells using 51153-1-AP (JNK antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HUVEC using 51153-1-AP

  Immunofluorescent analysis of HUVEC cells using 51153-1-AP (JNK antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, COS-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

51153-1-AP bindet in WB, IP, IHC, IF, ELISA JNK und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JNK fusion protein Ag1442
• Vollständiger Name: mitogen-activated protein kinase 9
• Berechnetes Molekulargewicht: 424 aa, 48 kDa
• Beobachtetes Molekulargewicht: 44-55 kDa
• GenBank-Zugangsnummer: BC032539
• Gene symbol: MAPK9
• Gene ID (NCBI): 5601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Publikationen

Species	Application	Title
human	WB	Adv Mater; m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation; Authors - Qing You
human	WB	Nat Commun; SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation.; Authors - Peiyuan Zhang
human	WB	Biomed Pharmacother; Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.; Authors - Ya-Sai Sun
rat	WB	Neurochem Res; Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK.; Authors - Xuehua Sun
rabbit	WB	Microb Pathog; Polysaccharides from Citrus grandis L. Osbeck suppress inflammation and relieve chronic pharyngitis.; Authors - Liyan Chen
mouse	IHC	J Ethnopharmacol; Integrated miRNA and mRNA omics reveal dioscin suppresses migration and invasion via MEK/ERK and JNK signaling pathways in human endometrial carcinoma in vivo and in vitro; Authors - Xiao-Li Li