MAPK12 Polyklonaler Antikörper

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• KD/KO Validated

MAPK12 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20184-1-AP

Synonyme

ERK 6, ERK3, ERK6, MAP kinase 12, MAP kinase p38 gamma, MAPK 12, MAPK12, P38GAMMA, PRKM12, SAPK 3, SAPK3

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Galerie der Validierungsdaten

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  WB analysis using 20184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20184-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20184-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 20184-1-AP

  IP Result of anti-MAPK12 (IP:20184-1-AP, 4ug; Detection:20184-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of mouse heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

20184-1-AP bindet in WB, IP, IHC MAPK12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAPK12 fusion protein Ag14073
• Vollständiger Name: mitogen-activated protein kinase 12
• Berechnetes Molekulargewicht: 367 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC015741
• Gene symbol: MAPK12
• Gene ID (NCBI): 6300
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Tumour Biol; Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.; Authors - Shutao Zheng ; KD Validated
rat	WB	BMC Endocr Disord; The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.; Authors - Shuman Wang