LC3C-Specific Polyklonaler Antikörper

LC3C-Specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18726-1-AP

Synonyme

LC3C, MAP1A/MAP1B LC3 C, MAP1A/MAP1B light chain 3 C, MAP1LC3C

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 18726-1-AP

  WB results of LC3C-specific antibody with Recombinant protein LC3A, LC3B and LC3C, by Dr. Christos Zois.

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  IHC staining of human liver using 18726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18726-1-AP (LC3C-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 18726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18726-1-AP (LC3C-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

18726-1-AP bindet in WB, IP, IHC, IF, ELISA LC3C-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: microtubule-associated protein 1 light chain 3 gamma
• Berechnetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: NM_001004343
• Gene symbol: MAP1LC3C
• Gene ID (NCBI): 440738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LC3C, also named as MAP1LC3C, belongs to the MAP1 LC3 family. It is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3C is produced by the proteolytic cleavage after the conserved C-terminal Gly residue. The antibody is specific to the prepeptide of LC3C.

Publikationen

Species	Application	Title
human	WB,IF	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely
human	WB,IP	Front Pharmacol; SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway; Authors - Dongheng Zhou
human	WB, IF	PLoS One; Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines.; Authors - Michael I Koukourakis
human	WB	Adv Exp Med Biol; Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina.; Authors - Anuradha Dhingra
human	WB	Dev Cell; V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation; Authors - Yiwei Sun