MAOB Monoklonaler Antikörper

MAOB Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B12F3

Kat-Nr. : 66107-1-Ig

Synonyme

MAO B, MAOB, monoamine oxidase B, Monoamine oxidase type B

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Galerie der Validierungsdaten

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  WB analysis of human brain using 66107-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 66107-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 66107-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human brain using 66107-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens).

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  IF Staining of mouse brain using 66107-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:40 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66107-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:40 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66107-1-Ig bindet in WB, IHC, IF, ELISA MAOB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAOB fusion protein Ag17918
• Vollständiger Name: monoamine oxidase B
• Berechnetes Molekulargewicht: 520 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC022494
• Gene symbol: MAOB
• Gene ID (NCBI): 4129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).