MAGEB18 Polyklonaler Antikörper

MAGEB18 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17494-1-AP

Synonyme

MAGE B18 antigen, MAGEB18, melanoma antigen family B, 18

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Galerie der Validierungsdaten

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  WB analysis using 17494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17494-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 17494-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17494-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 17494-1-AP

  Immunofluorescent analysis of A549 cells using 17494-1-AP (MAGEB18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A549-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17494-1-AP bindet in WB, IHC, IF,ELISA MAGEB18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAGEB18 fusion protein Ag11624
• Vollständiger Name: melanoma antigen family B, 18
• Berechnetes Molekulargewicht: 343 aa, 39 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC029525
• Gene symbol: MAGEB18
• Gene ID (NCBI): 286514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.