MAFB Polyklonaler Antikörper

MAFB Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20189-1-AP

Synonyme

KRML, Maf B, MAFB, Transcription factor MafB



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRAW 264.7-Zellen
Erfolgreiche Detektion in FCRAW 264.7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Durchflusszytometrie (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20189-1-AP bindet in WB, IP, FC, ELISA MAFB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MAFB fusion protein Ag14102
Vollständiger Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
Berechnetes Molekulargewicht 323 aa, 36 kDa
Beobachtetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerBC036689
Gene symbol MAFB
Gene ID (NCBI) 9935
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).

Protokolle

Produktspezifische Protokolle
WB protocol for MAFB antibody 20189-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Front Microbiol

Transcription factor MAFB controls type I and II interferon response-mediated host immunity in Mycobacterium tuberculosis-infected macrophages

Authors - Haruka Hikichi
  • KD Validated
humanWB

J Exp Clin Cancer Res

Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling

Authors - Weiwei Sheng
ratWB

Mol Cell Endocrinol

lnc-HC ameliorates steatosis by promoting miR-130b-3p biogenesis and the assembly of an RNA-induced silencing complex

Authors - Xi Lan
  • KD Validated
mouseWB,IP

Commun Biol

MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages

Authors - Huachun Cui