M-CSF Monoklonaler Antikörper

M-CSF Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G12E6

Kat-Nr. : CL488-65283

Synonyme

CSF 1, CSF1, Lanimostim, M CSF, MCSF

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using CL488-65283

  1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 405 Anti-Human CD11b then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65283

  1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65283 bindet in FC M-CSF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: M-CSF fusion protein HZ-1192
• Vollständiger Name: colony stimulating factor 1 (macrophage)
• Berechnetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC021117
• Gene symbol: CSF1
• Gene ID (NCBI): 1435
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)