Lysozyme Monoklonaler Antikörper

Lysozyme Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2G5A8

Kat-Nr. : 66456-1-Ig

Synonyme

1,4 beta N acetylmuramidase C, lysozyme, lysozyme (renal amyloidosis), Lysozyme C, LYZ, LZM

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Galerie der Validierungsdaten

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  WB analysis of human spleen using 66456-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 66456-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 66456-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66456-1-Ig

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, Hausschwein-Milzgewebe, Rattenmilzgewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66456-1-Ig bindet in WB, ELISA Lysozyme und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lysozyme fusion protein Ag6917
• Vollständiger Name: lysozyme (renal amyloidosis)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC004147
• Gene symbol: LYZ
• Gene ID (NCBI): 4069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia; Authors - Xin Xu