Ly-6G Monoklonaler Antikörper

Ly-6G Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1A8

Kat-Nr. : 65078-1-Ig

Synonyme

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G

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Galerie der Validierungsdaten

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  FC experiment of mouse bone marrow cells using 65078-1-Ig

  1X10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6G (65078-1-Ig, Clone:1A8) and FITC anti-Rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 65078-1-Ig

  1X10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6G (65078-1-Ig, Clone:1A8) and FITC anti-Rat IgG2a Antibody at dilution 1:100, or 0.5 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

65078-1-Ig bindet in IF, FC Ly-6G und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ly-6G-transfizierte EL-4J-Zelllinie
• Vollständiger Name: lymphocyte antigen 6 complex, locus G
• GenBank-Zugangsnummer: X70920
• Gene symbol: Ly6g
• Gene ID (NCBI): 546644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).

Publikationen

Species	Application	Title
mouse	IF	Eur J Pharmacol; Adelmidrol ameliorates liver ischemia-reperfusion injury through activating Nrf2 signaling pathway; Authors - Min Wu