LYN Monoklonaler Antikörper

LYN Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C7F2

Kat-Nr. : CL488-60211

Synonyme

FLJ26625, JTK8, LYN, Tyrosine protein kinase Lyn

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-60211

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 LYN antibody (CL488-60211, Clone: 3C7F2 ) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL488-60211

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 LYN antibody (CL488-60211, Clone: 3C7F2 ) at dilution of 1:200.

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  FC experiment of Ramos using CL488-60211

  1X10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LYN (CL488-60211, Clone:3C7F2) (red), or 0.8 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: Ramos-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60211 bindet in IF, FC (Intra) LYN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LYN fusion protein Ag12723
• Vollständiger Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Berechnetes Molekulargewicht: 512 aa, 59 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC075002
• Gene symbol: LYN
• Gene ID (NCBI): 4067
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.